Designing, Building, And Testing A Closed Compartment Stage Incubator, Ccsi
- Conference
- Location
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Chicago, Illinois
- Publication Date
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June 18, 2006
- Start Date
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June 18, 2006
- End Date
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June 21, 2006
- ISSN
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2153-5965
- Conference Session
- Tagged Division
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Instrumentation
- Page Count
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10
- Page Numbers
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11.422.1 - 11.422.10
- DOI
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10.18260/1-2--1176
- Permanent URL
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https://peer.asee.org/1176
- Download Count
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348
Paper Authors
Richard Hoehn Middle Tennessee State University
RICHARD HOEHN,
Mr. Hoehn is currently a graduate student, Computer Engineering Technology major at Middle Tennessee State University. Mr. Hoehn’s interests are wide spread in the technical field of PLC and PC-Based control applications. This also covers the design of embedded systems using off-the-shelf microcontrollers, CPLDs, and FPGAs. Mr. Hoehn is currently working for SME and is responsible for PLC and PC-Based instrumentation and control applications as well software development.
Saleh Sbenaty Middle Tennessee State University
SALEH M. SBENATY,
Dr. Sbenaty is currently a Professor of Engineering Technology at Middle Tennessee State University. He received the BS degree in EE from Damascus University and the MS and Ph.D. degrees in EE from Tennessee Technological University. He is actively engaged in curriculum development for technology education. He has written and co-authored several industry-based case studies. He is also conducting research in the area of mass spectrometry, power electronics, lasers, and instrumentation.
William Day Middle Tennessee State University
WILLIAM DAY,
Dr. Day is currently an Assistant Professor of Equine Science in the Department of Agribusiness Agriscience at Middle Tennessee State University. He received his Ph.D. in Reproductive Biology, his MS in Reproductive Physiology, and his BS in Animal Science all from Texas A&M. Dr. Day is the Director of the Equine Reproduction Laboratory at MTSU.
Abstract
NOTE: The first page of text has been automatically extracted and included below in lieu of an abstract
Designing, Building, and Testing a Closed Compartment Stage Incubator, CCSI
I. Introduction
The current paper describes the design, construction, and testing of a Closed Compartment Stage Incubator, CCSI. The CCSI was created in order to enable scientists in the Horse Science Laboratory at Middle Tennessee State University, MTSU, to monitor the growth of living cells under a microscope for extended periods. These experiments are referred to as time-course studies. Time-course studies must be performed under very specific and well-regulated conditions, which include controlled temperature, humidity, and ambient gases. The temperature of the CCSI must be maintained to a set value within ±0.1ºC degree over a period of as much as 10 hours or more. In addition, the gas mixture and humidity inside the CCSI must be concurrently controlled to enable the study and viewing of living cells under a microscope. Such an environment in which horse embryo cells can multiply and allows scientists to view this multiplication process under the microscope without impairing their growth is very desirable. The gas mixture, which is composed of N2 and CO2, is constantly circulated within a clear acrylic chamber to ensure even temperature, humidity, and gas distribution. The chamber and the control system are shown in Figure. 1.
This research is the direct result of an extensive collaboration between the author and his graduate advisor from the Department of Engineering Technology and Industrial Studies on one side and the Director of the Horse Science Laboratory at MTSU on the other. The initial research was Fig. 1. CCSI and its Controller. partially the result of an Undergraduate Research and Scholarly Creative Activities, URSCA, grant titled “Designing, Building, and Testing a Microcontroller-Based System for Industrial Applications.” The research has evolved into the current CCSI project at the graduate level. The authors believe to have found an inexpensive way that allows scientists to monitor the growth of living cells under the microscope. Similar systems are not readily available on the market or are far too expensive when custom made for smaller research facilities. This paper describes the process of designing, building, and testing of a Closed Compartment Stage Incubator. The paper also discusses the importance of a close collaboration, which ensures a high quality and successful research project, between two departments at MTSU; despite the fact that they are very different in nature.
II. The CCSI Design Process
The design of the CCSI project involved five stages. These were:
1. Defining System Specifications and Requirements.
Hoehn, R., & Sbenaty, S., & Day, W. (2006, June), Designing, Building, And Testing A Closed Compartment Stage Incubator, Ccsi Paper presented at 2006 Annual Conference & Exposition, Chicago, Illinois. 10.18260/1-2--1176
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