Brooklyn Technical High School, Brooklyn, New York, New York
October 26, 2018
October 26, 2018
October 27, 2018
Energy and cost-intensive conventional biological nitrogen removal (BNR) processes have historically been used to treat nitrogenous waste streams. The discovery of anaerobic ammonium oxidation (anammox) bacteria provided an alternative method of nitrogen removal that could significantly reduce carbon and oxygen requirements when combined with short-cut BNR processes. However, the enrichment of anammox bacteria can be challenging depending on waste stream and process characteristics. This student research study presents a mitigation technique by manipulating the hydraulic residence time (HRT) as a process control to enrich the anammox fraction of a bioreactor. This study is the result of a summer research internship that capitalized on a faculty-student connection in preparation for the students’ future semester-long capstone project.
The inherent risks with anammox bacteria are out-competition due to their slow growth rate and excessive nitrite (NO2-) inhibition or poisoning. Appropriate enrichment of an anammox fraction in relation to the influent characteristics ensures that anammox activity is high enough to effectively mitigate the impacts of excessive NO2-. Herein, the HRT was manipulated to provide sufficient time for the anammox reaction and prevent inhibitory conditions for anammox growth and enrichment. Anammox activity was measured via chemical analysis of nitrogen species. Molecular analysis was used to quantify anammox bacteria and enrichment was correlated to activity in terms of total inorganic nitrogen removal. With no direct requirements for exogenous carbon or oxygen, the incorporation of an enriched anammox fraction into a short-cut BNR system could significantly reduce a facility’s energy and chemical demands thus saving operational costs.
Brown, D. T., & Suh, J. Y., & Baideme, M. (2018, October), Impact of Hydraulic Residence Time on Anammox Enrichment: System Performance and Microbial Ecology Paper presented at 2018 Mid Atlantic Section Fall Meeting, Brooklyn Technical High School, Brooklyn, New York, New York. https://peer.asee.org/31454
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