Ebenstein, D. M., & Kennedy, E. A. (2012, June), A Cell Culture-based Biocompatibility Module for Biomedical Engineers  Paper presented at 2012 ASEE Annual Conference & Exposition, San Antonio, Texas. 10.18260/1-2--20781

\bibitem{asee_peer_20781}
  Ebenstein, D. M., \& Kennedy, E. A. (2012, June), \emph{A Cell Culture-based Biocompatibility Module for Biomedical Engineers}
  Paper presented at 2012 ASEE Annual Conference \& Exposition, San Antonio, Texas.
  10.18260/1-2--20781

Donna M. Ebenstein and Eric A. Kennedy.  "A Cell Culture-based Biocompatibility Module for Biomedical Engineers".  2012 ASEE Annual Conference & Exposition, San Antonio, Texas, 2012, June.  ASEE Conferences, 2012.  https://peer.asee.org/20781 Internet.  04 Dec, 2024

\bibitem{asee_peer_20781}
  Donna M. Ebenstein and Eric A. Kennedy.
  "A Cell Culture-based Biocompatibility Module for Biomedical Engineers".
  \emph{2012 ASEE Annual Conference \& Exposition, San Antonio, Texas, 2012, June}.
  ASEE Conferences, 2012.
  https://peer.asee.org/20781 Internet.  04 Dec, 2024

@INPROCEEDINGS{asee_peer_20781
author = "Donna M. Ebenstein and Eric A. Kennedy"
title = "A Cell Culture-based Biocompatibility Module for Biomedical Engineers"
booktitle = "2012 ASEE Annual Conference \& Exposition"
year = "2012"
month = "June"
address = "San Antonio, Texas"
publisher = "ASEE Conferences"
note = {https://peer.asee.org/20781}
number = {10.18260/1-2--20781}
}

TY  - CPAPER
AB  -    A Cell Culture-Based Biocompatibility Module for Biomedical EngineersAbstractThe challenge of exposing biomedical engineering students to the broad array of coreengineering and biology topics often makes it difficult to adequately address all relevant areas ofthe biomedical engineering field in the undergraduate curriculum. This paper will present a cell-culture based biocompatibility module with laboratory and lecture components that can be easilyintegrated into an engineering or biomaterials course.This module was developed to introduce students to basic cell culturing techniques and toprovide students with exposure to the issues related to the interaction between living and non-living materials (ABET Criterion 9). The lecture component focuses on biocompatibility issues,including: the definition of biocompatibility, cellular response to implantation of a foreignmaterial, and the types of biocompatibility tests recommended by the FDA. For the laboratorycomponent, students are first introduced to basic cell culture techniques, including steriletechnique, cell counting, and cell splitting. Next, students perform an in vitro cytotoxicity assay(direct contact) based on ISO-10993, gaining hands-on experience in biocompatibility testing.In its current form, the unit consists of 3 hours of lecture and 12 hours of cell culture lab spreadover 4.5 weeks; however, this unit can be shortened or expanded depending on time availableand the amount of supplementary topics introduced. A stand-alone cytotoxicity assay, forexample, could be completed by students in one week. Examples of supplemental activities thatcan be incorporated into the unit are included below:- Expanded group and individual cell culturing: After students learn and practice sterile     technique and cell culturing principles within small groups, each student is responsible to     split and maintain their own cell line for a period of time.- Training videos: To reinforce proper cell culturing practices, groups of students generate     training videos to demonstrate procedures such as good and bad pipetting practices,     performing a cell split, or counting cells.- Standards: The use of the ISO-10993 cytotoxicity assay opens the door for instruction on     the role of standards documents in defining accepted test procedures for medical device     development, and introduces the topic of standards organizations as a whole.- Statistics: Statistical methods can be reinforced by involving students in the design of the     experiments and requiring statistical analysis of quantifiable results of the cell culture     experiments, such as image analysis of the size and shape of cells or cell counts.- Bioethics: The use of HeLa cells or other cell lines originating from traceable human donors     affords an opportunity to discuss bioethics issues relating to biologic specimen collection.The self-contained biocompatibility module described in this paper provides biomedicalengineering students with relevant cell culture experience and exposure to the interactionsbetween living and non-living materials. A simple biocompatibility lecture-lab module can beincorporated into an existing class as a 1 or 2 week unit, or the length and depth of the unit canbe expanded through the incorporation of optional supplemental activities. Assessment resultsfrom the past three years demonstrate that the module enables students to meet biocompatibility-related performance criterion, and student satisfaction is indicated by positive comments fromstudent course evaluations.
AU  - Donna M. Ebenstein
AU  - Eric A. Kennedy
CY  - San Antonio, Texas
DA  - 2012/06/10
PB  - ASEE Conferences
TI  - A Cell Culture-based Biocompatibility Module for Biomedical Engineers
UR  - https://peer.asee.org/20781
DO  - 10.18260/1-2--20781
ER  -