Conference Session

Materials Division Technical Session 3

Collection

2017 ASEE Annual Conference & Exposition

Authors

Hui Shen, Ohio Northern University; Vishal R. Mehta, Ohio Northern University

Tagged Divisions

Materials

. Scott Cottle, the machinist at Ohio Northern University.Bibliography[1] Núñez J. S., Lascano S. F., Esparragoza, I. E. A project-based learning approach for a first-year engineering course, Eleventh LACCEI Latin American and Caribbean Conference forEngineering and Technology (LACCEI’2013), Cancun Mexico, August 14 - 16, 2013.[2] McLoone, S.C., Lawlor, B. J., and Meehan, A. R. The Implementation and Evaluation of aProject-Oriented Problem-Based Learning Module in a First Year Engineering Program. Journalof Problem based learning in Higher Education, 4(1), 2016.[3] Anwar, S and Granlund, E. Enhancing a Freshman Level Engineering Design Course throughProject Based Learning. Proceedings of the 2003 American Society for Engineering EducationAnnual

Conference Session

Makerspaces

Collection

2018 ASEE Annual Conference & Exposition

Authors

Hoda Ehsan, Purdue University, West Lafayette; Tikyna Dandridge, Purdue University, West Lafayette; Ibrahim H. Yeter, Purdue University, West Lafayette; Monica E. Cardella, Purdue University, West Lafayette

Tagged Divisions

Pre-College Engineering Education

, Senay Purzer, Anastasia Rynearson, Cetin Biller,Kayla Carter, Jessica Rush Leeker and Terri Sanger. References[1] C. M. Cunningham, “Engineering is elementary: An engineering and technology curriculum for children,” Eng. Educ., 2007.[2] H. A. Diefes-Dux, M. Hjalmarson, T. Miller, and R. Lesh, “Model eliciting activities for engineering education,” in Models and modeling in engineering education: Designing experiences for all students, J. S. Zawojewski, H. Diefes-Dux, and K. Bowman, Eds. Rotterdam: Sense Publishers, 2008, pp. 17–36.[3] Ş. Purzer, M. H. Goldstein, R. S. Adams, C. Xie, and S. Nourian, “An exploratory study of informed engineering design behaviors

Conference Session

Accreditation and the BOK

Collection

2018 ASEE Annual Conference & Exposition

Authors

Angela R. Bielefeldt, University of Colorado, Boulder

Tagged Divisions

Civil Engineering

13 13 18 11 4.5 A 2. Natural sciences 3 36 5 4.5A 3. Humanities 2 77 1 S -- 4. Social sciences 2 69 2 I -- 5. Material science 8 15 12 -- 6. Mechanics 14 12 11 13 -- 7. Experiments 3 41 3 S 3.7 8. Problem

Conference Session

First-year Programs Division Postcard Session 1: Retention and Student Success Strategies

Collection

2018 ASEE Annual Conference & Exposition

Authors

Emily Knaphus-Soran, University of Washington; Ann Delaney, Boise State University; Katherine Christine Tetrick, Washington State University; Sonya Cunningham, University of Washington; Pamela Cosman, University of California, San Diego; Tanya D. Ennis, University of Colorado, Boulder; Beth A. Myers, University of Colorado, Boulder; Jana Milford, University of Colorado, Boulder; Donna C. Llewellyn, Boise State University; Eve A. Riskin, University of Washington; Janet Callahan, Boise State University; Kevin Pitts, University of Illinois, Urbana-Champaign; Michelle Ferrez, University of California, San Diego

Tagged Topics

Diversity

Tagged Divisions

First-Year Programs

aimed at improvingthe engagement, retention, and graduation of students underrepresented in engineering. Thesecomponents include: “intrusive” academic advising and support services, intensive first-yearacademic curriculum, community-building (including pre-matriculation summer programs),career awareness and vision, faculty mentorship, NSF S-STEM scholarships, and second-yearsupport.This work in progress paper describes the implementation of the Redshirt program2 at each of thesix Redshirt in Engineering Consortium institutions, providing a variety of models for how an1 For brevity, we will use the acronyms listed in this table in place of the full names of theinstitutions throughout the paper.extra preparatory year or other intensive academic

Conference Session

Track : Special Topics - Identity Technical Session 8

Collection

2019 CoNECD - The Collaborative Network for Engineering and Computing Diversity

Authors

Stephen Secules, Purdue University, West Lafayette ; Cassandra J. Groen-McCall, Virginia Tech

Tagged Topics

Diversity, Special Topic: Identity

Conference Session

Women in Engineering Division Technical Session 5

Collection

2019 ASEE Annual Conference & Exposition

Authors

Bradley Joseph Priem, Northeastern University; Caroline Ghio, Northeastern University; Hannah Boyce, Northeastern University; Sydney Anne Morris, Northeastern University; Emma Kaeli, Stanford University; Tyler Byrne Cole, Northeastern University; Paul A. DiMilla, Northeastern University; Rachelle Reisberg, Northeastern University

Tagged Topics

Diversity

Tagged Divisions

Women in Engineering

Conference Session

ENT Division Technical Session: EM Across the Curriculum I

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Kevin D. Dahm, Rowan University; Scott Duplicate Streiner, Rowan University; Cheryl A. Bodnar, Rowan University; Kaitlin Mallouk, Rowan University; Bruce Oestreich, Rowan University; Ted Howell, Rowan University; Jennifer Tole, Rowan University

Tagged Divisions

Entrepreneurship & Engineering Innovation

team re-designed each of these three major coursedeliverables, with the goal of fostering an Entrepreneurial Mindset in students and leveragingsynergies between the Entrepreneurial Mindset and the existing goals of the course (engineeringdesign and technical communication). In particular, the faculty team created a new linkagebetween the research sequence and the humanities assignment. The new research sequence isbuilt around the U.N.’s Sustainable Development Goals; each student chooses one of the goals toexplore through their individual rhetorical analysis, annotated bibliography, and literaturereview. The humanities assignment is a team project in which students explore solutions tosustainability problems on the campus of Rowan University

Conference Session

NSF Grantees: Student Thought

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Andrew Olewnik, State University of New York at Buffalo; Randy K. Yerrick, State University of New York at Buffalo; Manoj Madabhushi; Rachith R. Ramaswamy, State University of New York at Buffalo; Yonghee Lee, State University of New York at Buffalo; Hala Alfadhli, State University of New York at Buffalo; Amanda A. Simmons, State University of New York at Buffalo

Tagged Topics

NSF Grantees Poster Session

problem solvers from poor ones by their awareness of which strategies theyhave used and their knowledge of where they are in their thinking relative to the final solution[18].Such criticisms have led some to back away from the “teaching problem solving” approachemerging from the 1970’s [19–21]. In fact, Schön went as far to argue that there is no such thingas problem solving in the engineering profession as “no engineer has ever been given a problemto solve.” Schön’s contention is the value of engineers’ work is not found in their problemsolving abilities. Rather, the essential facet of engineers’ work is found in their “problemsetting.” Engineers make sense of a given messy world from which many factors need to beconsidered, organized, and

Conference Session

Mental Health of Graduate Students

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Amanda C. Arnold, Arizona State University; Kerrie G. Wilkins-Yel, University of Massachusetts Boston; Jennifer M. Bekki, Arizona State University; Bianca L. Bernstein, Arizona State University; Madison Natarajan, University of Massachusetts Boston; Ashley K. Randall, Arizona State University ; Roxanna Francies, Arizona State University; Chinwendu Elyse Okwu, University of Pittsburgh

Tagged Topics

Diversity

Tagged Divisions

Graduate Studies

herself, "This is really mentally affecting me." Erin noticed thetoll that graduate school had on her mental health and attributed this to the lack of preparationon the part of advisors and mentors. She stated, I had realized the mental and emotional toll that grad school was heaping on [me], honestly, unrealistic level[s] of expectations, the multiple projects, and the teaching, and still dealing with personal life, and all while being thrown in the deep end. No one actually ever teaches you how to do research. You just kind of sink or swim.Giselle’s increased mental distress led to her decision to take a leave of absence. She shared, I had to take a leave of absence, because I couldn't deal with it. I had to take a

Conference Session

Instruments and Methods for Studying Student Experiences and Outcomes

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

David Reeping, Virginia Polytechnic Institute and State University; Cherie D. Edwards, Virginia Commonwealth University

Tagged Divisions

Educational Research and Methods

] Criterion Description Amount of Mixing There should be nontrivial, meaningful mixing in a mixed methods publication, else the study would be better classified as multi-method. This criterion spotlights methodologists’ attention to integration in mixed methods research [see 1]. Interpretive Interpretive comprehensiveness refers to how the researcher(s) Comprehensiveness engage different perspectives in their study. This can be accomplished throughout the design by picking extreme or negative cases, testing competing hypotheses, and

Conference Session

College Industry Partnerships Division Technical Session 3

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Hang Zhang, Beihang University; Ming Li, Beijing Foreign Studies University

Tagged Topics

Diversity

Tagged Divisions

College Industry Partnerships

Conference Session

Flipped, Blended, Online, Oh My

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Kimberly Warren, University of North Carolina at Charlotte; Meagan Padro, University of North Carolina at Charlotte; Chuang Wang, University of Macau

Tagged Divisions

Civil Engineering

Likert Taxonomy Criteria Scale Level(a) 4 Examine correct equation for d(min) 3 Interpret d(max) 0 = No work 3 Execute the equation 1 Report units 1 = Method and/or understanding(b) 4 Examine correct zero air void line significantly below standard equation 1 Remember to use specific gravity in 2 = Touches on right method but equation significant errors in concept 3 Interpret optimum water content 2 Identify S = 1.0 for the zero air void 3

Conference Session

Multidisciplinary Service and Outreach Projects

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Kristie Gutierrez, Old Dominion University; Stacie I. Ringleb, Old Dominion University ; Jennifer Jill Kidd, Old Dominion University; Orlando M. Ayala, Old Dominion University; Pilar Pazos, Old Dominion University; Krishnanand Kaipa, Old Dominion University

Tagged Topics

Diversity

Tagged Divisions

Multidisciplinary Engineering

Conference Session

Women in Engineering Division Technical Session 8

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Robin O. Andreasen, University of Delaware; Shawna Vican, University of Delaware; Yvette A Jackson, University of Massachusetts Dartmouth

Tagged Topics

Diversity

Tagged Divisions

Women in Engineering

Conference Session

Mechatronics and Robotics II

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Nima Lotfi, Southern Illinois University, Edwardsville; Kenechukwu Churchill Mbanisi, Worcester Polytechnic Institute; David M. Auslander, University of California, Berkeley; Carlotta A. Berry, Rose-Hulman Institute of Technology; Luis Alberto Rodriguez, Milwaukee School of Engineering; Majid Molki, Southern Illinois University, Edwardsville

Tagged Divisions

Multidisciplinary Engineering

, tutorials and documentationdeveloped by MRE faculty can significantly help with widespread use and adoption of open-sourceplatforms in higher education institutions. 12References[1] Laurent, A. M. S. (2004). Understanding open source and free software licensing: guideto navigating licensing issues in existing & new software. "O'Reilly Media, Inc.".[2] Open Source Hardware Association (OSHWA). Brief History of Open Source Hardware:Organizations and Definitions. https://www.oshwa.org/research/brief-history-of-open-source-hardware-organizations-and-definitions/ [accessed December 2019][3] OpenSource.com. What are Open Hardware. https://opensource.com/resources/what-open-hardware [accessed

Conference Session

Student Division Technical Session 2

Collection

2020 ASEE Virtual Annual Conference Content Access

Authors

Atsushi Akera, Rensselaer Polytechnic Institute; Soheil Fatehiboroujeni, Purdue University, West Lafayette; Sarah Appelhans, University at Albany-SUNY; Joerene Acerrador Aviles, Rensselaer Polytechnic Institute; Eva Dibong; Beatrice Mendiola, Rensselaer Polytechnic Institute; Michelle Murray, Rensselaer Polytechnic Institute; Melissa Shuey, Rensselaer Polytechnic Institute; Marta Tsyndra, Rensselaer Polytechnic Institute; Makayla Wahaus, Rensselaer Polytechnic Institute

Tagged Topics

Diversity

Tagged Divisions

Student

especially true at public institutions driven toexpand access while improving retention rates, based on performance metrics set by the state.Retention studies have been conducted for nearly every sub-population including women andmany racial and ethnic groups. Some of the work has shifted to intersectional analyses—forexample, Archer’s exploration of black male students’ resistance to “geeky” identities [10], orJohnson et al.’s study which highlights some Native American and Latina women’s preference towork as scientists within their ethnic communities as a method of balancing ethnic andengineering identities [11]. However, less work has been done on the interactions that occuracross different student cohorts. Indeed, scholars have argued that due

Conference Session

NSF Grantees Poster Session

Collection

2007 Annual Conference & Exposition

Authors

Nathan Klingbeil, Wright State University; Kuldip Rattan, Wright State University; Michael Raymer, Wright State University; David Reynolds, Wright State University; Richard Mercer, Wright State University; Anant Kukreti, University of Cincinnati; Brian Randolph, University of Toledo

AC 2007-1116: A NATIONAL MODEL FOR ENGINEERING MATHEMATICSEDUCATIONNathan Klingbeil, Wright State University Nathan W. Klingbeil is an Associate Professor of Mechanical Engineering and Robert J. Kegerreis Distinguished Professor of Teaching at Wright State University. He is the lead PI for WSU's National Model for Engineering Mathematics Education. He is the recipient of numerous awards for his work in engineering education, including the CASE Ohio Professor of the Year Award (2005) and the ASEE North Central Section Outstanding Teacher Award (2004).Kuldip Rattan, Wright State University Kuldip S. Rattan is a Professor in the Department of Electrical Engineering at Wright State University

Conference Session

Professional Graduate Education & Industry Duplicate Session

Collection

2006 Annual Conference & Exposition

Authors

Duane Dunlap, Western Carolina University; John Bardo, Western Carolina University; Donald Keating, University of South Carolina; Thomas Stanford, University of South Carolina; Eugene DeLoatch, Morgan State University; Albert McHenry, Arizona State University; Joseph Tidwell, Arizona State University; Niaz Latif, Purdue University; Mark Schuver, Purdue University; Dennis Depew, Purdue University; Roger Olson, Rolls-Royce; David Quick; Stephen Tricamo, New Jersey Institute of Technology; Samuel Truesdale, Rolls-Royce; Jay Snellenberger, Rolls-Royce; Harvey Palmer, Rochester Institute of Technology; Mohammad Noori, North Carolina State University; Kathleen Gonzalez Landis, University of Arizona; Ronald Bennett, University of St. Thomas

,Engineering, and Public Policy (COSEPUP)3 has pointed out, graduate education in engineeringhas evolved primarily in the United States as a byproduct of a national science policy forscientific research. The United States has not had a coherent policy for the graduate developmentof the vast majority of its domestic engineering graduates in the U.S. engineering workforcewhose professional careers are not centered on academic scientific research but rather arecentered on creating, developing, and innovating new, improved, and breakthrough technologyin industry for competitiveness and the nation’s defense.Whereas the nation invested heavily during the 1960’s, 70’s, 80’s, and 90’s in the graduate

Conference Session

NSF Grantees' Poster Session

Collection

2013 ASEE Annual Conference & Exposition

Authors

Alan D. Niemi, LeTourneau University; Matthew G. Green, LeTourneau University; Melanie Roudkovski, LeTourneau University

Tagged Topics

NSF Grantees Poster Session

Increase the number of Introduction to engineers in the Engineering Increase SEET workforce Practice II (S) student motivation Industrial Mentors to study (IM) engineering at LETU Institutionalize

Conference Session

Tricks of the Trade

Collection

2013 ASEE Annual Conference & Exposition

Authors

Katy Luchini-Colbry, Michigan State University; Tobias Ortega-Knight, University of the Virgin Islands; Charles Huang Chen, Michigan State University; Danny Lynch, University of the Virgin Islands; Kathleen Anne Fitzsimons, Michigan State University; Crystal D Alton; Juan L. Mena Lapaix, Michigan State University; Joshua Drost, Michigan State University; Garrett Kohler

Tagged Divisions

Student

Conference Session

Community Engagement Division Poster Session

Collection

2013 ASEE Annual Conference & Exposition

Authors

Bowa George Tucker, UMass Lowell; David O Kazmer, University of Massachusetts, Lowell; Olga Pierrakos, James Madison University; Chris Swan, Tufts University; Angela R Bielefeldt, University of Colorado, Boulder; Kurt Paterson P.E., Michigan Technological University; Annie Soisson, Tufts University

Tagged Divisions

Community Engagement Division

’ mastery of course content as a result of LTS is as evident as withservice-learning. Further analysis and investigation is required to extract and validate theprimary themes as a function of respondent demographics towards the development ofappropriate recommendations and policies.Bibliography1 National Science Foundation (NSF) 2007 Moving Forward to Improve Engineering Education(No NSB-07-122) Washington, DC: National Science Foundation.2 Lathem, S. A., NEUMANN, M. D., & Hayden, N. (2011). The Socially Responsible Engineer:Assessing Student Attitudes of Roles and Responsibilities. Journal of Engineering Education,100(3), 444-474.3 Vanasupa, L., et al. (2006) Global Challenges as Inspiration: A Classroom strategy to fostersocial responsibility

Conference Session

ECE Curriculum Improvement

Collection

2013 ASEE Annual Conference & Exposition

Authors

Tatiana V. Goris, Purdue University, West Lafayette; Michael J. Dyrenfurth, Purdue University, West Lafayette

Tagged Divisions

Electrical and Computer

not recognized early in the course, thestudent will not only fail to understand much of the new material, but worse, s/he is likely todress up his misconceptions in scientific jargon, giving the false impression that s/he has learnedsomething about science” ( [4], p. 1048).The other unexpected result was that despite of significant improvement in understanding ofelectricity, seniors were more confused than novices about physical and fundamental electricalphenomena such as charge, electrical field and current. Students’ responses to the DIRECTconcept inventory were similar to research outcomes of the original author of the DIRECT test, Page

Conference Session

INT. Engineering Education: Developments, Innovations, Partnerships, and Implementations

Collection

2013 ASEE Annual Conference & Exposition

Authors

James L. Barnes, James Madison University; Michael J. Dyrenfurth, Purdue University, West Lafayette; Susan Kubic Barnes, James Madison University

Tagged Divisions

International

Understanding that change and variation rule everything in naturecNote. a Adapted from Biomimetics: Technology imitates nature, by H. Yahya, 2006, Istanbul: Global Publishing. b Adapted fromAn epistemological problem-centric approach to solving complex global problems, paper presented by J. Barnes, S. Barnes, andM. Dyrenturth at the Forum on Philosophy, Engineering & Technology (fPET-2012), The Graduate University of the ChineseAcademy of Sciences (GUCAS), Beijing, People’s Republic of China. Manuscript submitted for publication. c Adapted fromLearning from the octopus: How secrets from nature can help us fight terrorist attacks, natural disasters, and disease by R

Conference Session

Programs in Support of Systems Engineering Education

Collection

2013 ASEE Annual Conference & Exposition

Authors

Chet Boncek Jr, Raytheon Company

Tagged Divisions

Systems Engineering

Domain/Topic Grade/StrandFramework Figure(s) Significance to 'Power Power to Grades Code Text of The Standard

Conference Session

Training and Mentoring of Graduate Teaching Assistants

Collection

2013 ASEE Annual Conference & Exposition

Authors

Tershia A. Pinder-Grover, University of Michigan

Tagged Divisions

Graduate Studies

thisterm?”The majo ority of TAs (64%) who reported usiing active le arning indiccated that theey were onlyy“somewh hat successfuul” implemennting these strategies s in their class, 12% reporteed being “verrysuccessfuul,” while 17 7% reported being “unsu uccessful” annd 7% weree “very unsucccessful” (Fiigure2). For thhis question, only 14 TA As provided further explaanation. Sinnce the majoority of thecommentts were from m “somewhatt successful”” and “very ssuccessful” T TAs, these ccommentscentered around TAss receiving positive p studeent feedbackk (N=11), annd recognizinng students bbeingable to

Conference Session

Understanding Students: Cognition

Collection

2003 Annual Conference

Authors

F. Lee Grismore; Dr. Juliet Hurtig; Dr. Barry Farbrother

of bachelor degrees is less than thevalue of Nreq, a positive difference signal is formed and feeds back into the system in such a way asto increase the fraction of students that remain on the engineering pathway. Ks stands for “Schoolsystem input coefficient” and K c stands for “College system input coefficient”. Each of these Page 8.180.2coefficients is the sum of two terms. The functional relationship for Ks is“Proceedings of the 2003 American Society for Engineering Education Annual Conference & Exposition Copyright  2003, American Society for Engineering Education” K s

Conference Session

Engineering and Public Policy II

Collection

2012 ASEE Annual Conference & Exposition

Authors

Mike Murphy, Dublin Institute of Technology; Michael J. Dyrenfurth, Purdue University, West Lafayette

Tagged Divisions

Engineering and Public Policy

in industry, while Pavitt (1998) describes such research as “augmenting the capacity of business to solve complex problems.” See N. Rosenberg, and R. R. Nelson, “American universities and technical advance in industry,” Research   Policy,   23:323-348, 1994. See also K. Pavitt, “The Social Shaping of the National Science Base,” Research  Policy,  27:793-805, 1998. 34Y. S. Lee, “The Sustainability of University-Industry Research Collaboration,” Journal   of   Technology   Transfer, 25(2), 2000. 35Bronwyn Hall, “University-Industry Research Partnerships in the United States,” Kansai Symposium Paper, February 2004. 36Some analysts point out, however, that not all universities are structured

Conference Session

Computers and Simulation

Collection

2012 ASEE Annual Conference & Exposition

Authors

Hatem M. Wasfy, Advanced Science and Automation Corp.; Tamer M. Wasfy, Indiana University-Purdue University, Indianapolis; Jeanne Peters, Advanced Science and Automation Corp.

Tagged Divisions

Computers in Education

: Page 25.641.7  cp d& d& ≥ 0 Fnormal = A kp d + A (21) & d& < 0  s p cp d d& = vn i ni (22) Contact surface r Contact n point

Conference Session

NSF Grantees' Poster Session

Collection

2012 ASEE Annual Conference & Exposition

Authors

Ashland O. Brown P.E., University of the Pacific; Daniel D. Jensen, U.S. Air Force Academy; Joseph J. Rencis P.E., Tennessee Technological University; Kristin L. Wood, Singapore University of Technology and Design; Kyle A. Watson, University of the Pacific; Chuan-Chiang Chen, California State Polytechnic University, Pomona; Vladimir A. Labay, Gonzaga University; Ismail I. Orabi, University of New Haven; Firas Akasheh, Tuskegee University; John J. Wood, U.S. Air Force Academy; Rachelle Kisst Hackett, University of the Pacific; Kathy Schmidt Jackson, Pennsylvania State University; Jiancheng Liu, University of the Pacific; Paul Henry Schimpf; Alexis Pham, University of the Pacific; Krystian Zimowski, University of Texas, Austin; Kristin Taylor, University of the Pacific

Tagged Topics

NSF Grantees Poster Session

TypeThe Myers Briggs Type Indicator (MBTI) is similar to Felder-Silverman Learning Style, but islinked to personality preferences as shown in Table 2. MBTI includes four categories of how anindividual processes and evaluates information.29 The first category describes how a personinteracts with his or her environment. People who take initiative and gain energy frominteractions are known as Extroverts (E). Introverts (I), on the other hand prefer more of arelatively passive role and gain energy internally. The second category describes how a personprocesses information. People who process data with their senses are referred to as Sensors (S),and a person who sees where data is going in the future is called an iNtuitor (N). The Sensorversus

Conference Session

Best. Class. Ever.

Collection

2012 ASEE Annual Conference & Exposition

Authors

Bernard J. Van Wie, Washington State University; David B. Thiessen, Washington State University; Marc Compere, Embry-Riddle Aeronautical Univ., Daytona Beach FL; Ximena Toro; Jennifer C Adam, Washington State University; Shane A. Brown P.E., Washington State University; Andrew P Easley, Washington State University; Xuesong Li P.E., Washington State University; Kevin Lee, University of Idaho; Mert Colpan, Washington State University; Kevin Tyler Gray, Washington State University; Benjamin Garrett, Washington State University; Shane Riley Reynolds, Washington State University; Paul B. Golter, Washington State University; Olusola Adesope, Washington State University, Pullman

Tagged Divisions

Chemical Engineering

collected to confirm that biotin-FITC could be bound to streptavidincoated 96-well plates. Data plotted on a fluorescence vs. log of concentration graph show thetypical S-shaped curve for adsorbed species with a minimum detection limit of 50 μM and max-imum value of 5 mM of Biotin-FITC. The next experiment was to determine if the biotin-inhibitor could capture the DAPI-LNCaPs on the coated plastic surfaces. DAPI or 4’6-diamidino-2-phenylindole is a fluorescent stain that binds strongly to A-T rich regions in DNAthat excites at 358 nm and emits at 461 nm. Preliminary results suggest when more PCTCs are insuspension there is indeed a higher fluorescence intensity than less concentrated suspensions andboth high and low suspension concentrations result